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293f-expressed recombinant ha proteins  (eEnzyme Inc)

 
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    eEnzyme Inc 293f-expressed recombinant ha proteins
    293f Expressed Recombinant Ha Proteins, supplied by eEnzyme Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/293f-expressed+recombinant+ha+proteins/293f+expressed+recombinant+ha+proteins/bio_rxiv__2024__10__10__617255-135-1-13
    Average 90 stars, based on 1 article reviews
    293f-expressed recombinant ha proteins - by Bioz Stars, 2026-10
    90/100 stars

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    Recombinant:

    Article Title: mRNA-based influenza vaccine expands breadth of B cell response in humans
    Article Snippet: For multiplex fluorescent bead assay, 293F-expressed recombinant HA proteins from the following influenza strains were purchased from Immune Technology Corp: H3N2 strains, A/Thailand/8/2022, A/Darwin/6/2021, A/Cambodia/e0826360/2020, A/South Australia/34/2019, A/Hong Kong/2671/2019, A/Singapore/INFIMH-16-0019/2016, A/Switzerland/8060/2017, A/Victoria/361/2011, A/Brisbane/10/2007; H1N1 strains, A/Wisconsin/67/2022, A/Sydney/5/2021, A/Wisconsin/588/2019, A/Guangdong-Maonan/SWL1536/2019, A/Michigan/45/2015, A/California/04/2009, A/Puerto_Rico/8/1934. .. Additionally, 293F-expressed recombinant HA proteins from the following influenza strains were purchased from eEnzyme LLC: H3N2 strains, A/Texas/50/2012, A/Perth/16/2009, A/Hong Kong/1/1968; H1N1 strains, A/Hawaii/70/2019, A/Brisbane/2/2018. ..



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    a , HA proteins encoded by mRNA-1010 for the 2022-2023 Northern Hemisphere influenza vaccine strains. Fluarix vaccine included the same strains, except H3N2 was egg-based (A/Darwin/9/2021). b , ELISpot quantification of mean HA-binding IgM-secreting PBs in blood at baseline, 1, and 2 weeks post vaccination in mRNA-1010 (red) and Fluarix (black) participants. Numbers of HA-specific PBs were quantified against the four vaccine HAs and averaged. c , ELISpot quantification of HA-binding IgM-secreting PBs at 1 week post vaccination in mRNA-1010 (red) and Fluarix (black) participants. Horizontal bars represent geometric means. P values determined by Mann-Whitney U test. d , Flow cytometry gating strategy for HA-specific MBCs from PBMCs e , Quantification of HA-specific MBCs as a percentage of CD19+ cells in blood by flow cytometry at baseline, 4, and 17/26 weeks post vaccination in mRNA-1010 (red) and Fluarix (black) participants. Numbers represent geometric mean frequencies of MBCs for each time point. f , Fold change in HA-specific MBCs as a percentage of CD19+ cells in blood by flow cytometry at indicated time points over week 0 for mRNA-1010 (red) and Fluarix (black) participants. Numbers represent mean values. In e and f , P values determined by Mann-Whitney U test. For participants that did not complete a blood collection at week 26 or PBMCs were not available, samples from 17 weeks post vaccination were used for analysis (mRNA-1010, n=3; Fluarix, n=2).

    Journal: bioRxiv

    Article Title: mRNA-based influenza vaccine expands breadth of B cell response in humans

    doi: 10.1101/2024.10.10.617255

    Figure Lengend Snippet: a , HA proteins encoded by mRNA-1010 for the 2022-2023 Northern Hemisphere influenza vaccine strains. Fluarix vaccine included the same strains, except H3N2 was egg-based (A/Darwin/9/2021). b , ELISpot quantification of mean HA-binding IgM-secreting PBs in blood at baseline, 1, and 2 weeks post vaccination in mRNA-1010 (red) and Fluarix (black) participants. Numbers of HA-specific PBs were quantified against the four vaccine HAs and averaged. c , ELISpot quantification of HA-binding IgM-secreting PBs at 1 week post vaccination in mRNA-1010 (red) and Fluarix (black) participants. Horizontal bars represent geometric means. P values determined by Mann-Whitney U test. d , Flow cytometry gating strategy for HA-specific MBCs from PBMCs e , Quantification of HA-specific MBCs as a percentage of CD19+ cells in blood by flow cytometry at baseline, 4, and 17/26 weeks post vaccination in mRNA-1010 (red) and Fluarix (black) participants. Numbers represent geometric mean frequencies of MBCs for each time point. f , Fold change in HA-specific MBCs as a percentage of CD19+ cells in blood by flow cytometry at indicated time points over week 0 for mRNA-1010 (red) and Fluarix (black) participants. Numbers represent mean values. In e and f , P values determined by Mann-Whitney U test. For participants that did not complete a blood collection at week 26 or PBMCs were not available, samples from 17 weeks post vaccination were used for analysis (mRNA-1010, n=3; Fluarix, n=2).

    Article Snippet: Seasonal quadrivalent mRNA-1010 vaccine (Northern Hemisphere 2022-2023 season) was provided by Moderna, Inc. For ELISpot, 293F-expressed recombinant HA proteins derived from H1N1 (A/Wisconsin/588/2019), H3N2 (A/Darwin/6/2021), B/Yamagata/16/88-like lineage (B/Phuket/3073/2013), or B/Victoria/2/87-like lineage (B/Austria/1359417/2021) were provided by Moderna, Inc. For ELISA, 293F-expressed recombinant HA proteins were provided by Moderna, Inc. (A/Wisconsin/588/2019, B/Phuket/3073/2013) or purchased from SinoBiological (A/Darwin/6/2021, B/Austria/1359417/2021).

    Techniques: Northern Blot, Enzyme-linked Immunospot, Binding Assay, MANN-WHITNEY, Flow Cytometry

    a , Phylogenetic tree of influenza strains. H1N1 strains used for bead assay are highlighted in orange; H3N2 strains are highlighted in blue. b , Fold change in MFI of binding of plasma samples at 4 weeks over baseline for mRNA-1010 (red) and Fluarix (black) participants to beads coated in A/H1 glycoproteins. c , Fold change in MFI of binding of plasma samples at 17/26 weeks over baseline for mRNA-1010 (red) and Fluarix (black) participants to beads coated in A/H1 glycoproteins. Samples collected at 17 weeks post vaccination were used for patients that did not complete a blood draw at 26 weeks (mRNA-1010, n=2, Fluarix, n=2). For b and c, P values were determined by Mann-Whitney U test; bars represent median values.

    Journal: bioRxiv

    Article Title: mRNA-based influenza vaccine expands breadth of B cell response in humans

    doi: 10.1101/2024.10.10.617255

    Figure Lengend Snippet: a , Phylogenetic tree of influenza strains. H1N1 strains used for bead assay are highlighted in orange; H3N2 strains are highlighted in blue. b , Fold change in MFI of binding of plasma samples at 4 weeks over baseline for mRNA-1010 (red) and Fluarix (black) participants to beads coated in A/H1 glycoproteins. c , Fold change in MFI of binding of plasma samples at 17/26 weeks over baseline for mRNA-1010 (red) and Fluarix (black) participants to beads coated in A/H1 glycoproteins. Samples collected at 17 weeks post vaccination were used for patients that did not complete a blood draw at 26 weeks (mRNA-1010, n=2, Fluarix, n=2). For b and c, P values were determined by Mann-Whitney U test; bars represent median values.

    Article Snippet: Seasonal quadrivalent mRNA-1010 vaccine (Northern Hemisphere 2022-2023 season) was provided by Moderna, Inc. For ELISpot, 293F-expressed recombinant HA proteins derived from H1N1 (A/Wisconsin/588/2019), H3N2 (A/Darwin/6/2021), B/Yamagata/16/88-like lineage (B/Phuket/3073/2013), or B/Victoria/2/87-like lineage (B/Austria/1359417/2021) were provided by Moderna, Inc. For ELISA, 293F-expressed recombinant HA proteins were provided by Moderna, Inc. (A/Wisconsin/588/2019, B/Phuket/3073/2013) or purchased from SinoBiological (A/Darwin/6/2021, B/Austria/1359417/2021).

    Techniques: Binding Assay, MANN-WHITNEY